Investigational techniques – Examination of nociception

1. Behavioural tests:

  • observation of the nocifensive behaviours such as paw lifting and licking (formalin test, PMA test) as well as writhing movements; light aversion test in migraine models

2. Thermonociceptive tests:

  • Plantar test (Life Sciences)
  • Increasing temperature hot plate (ITHP): our novel method

    • excellent reproducibility of the behavioural noxious heat threshold
    • high sensitivity for measuring the antinociceptive and antihyperalgesic effects of both conventional and non-conventional analgesics (Almasi et al. Br. J. Pharmacol. 2003.)
  • Increasing temperature water bath (ITWB): our novel method (Bolcskei et al. Eur. J. Pharmacol. 2007.)

3. Mechanonociceptive tests

  • Ugo Basile Analgesimeter (touch sensitivity measurement)
  • Ugo Basile Dynamic Plantar Aesthesiometer (mechanonocieption, paw pressure measurement)

4. Examination of weight bearing

  • Dynamic weight bearing (Bioseb)

Examination of anxiety, depression-like behaviors, learning and memory (mouse behavioral phenotyping)

  1. Open Field (spontaneous locomotor activity)
  2. Light-dark box (anxiety)
  3. Elevated Plus maze (anxiety)
  4. Tail suspension test (depression-like behavior)
  5. Forced swim test (depression-like behavior)
  6. Morris Water Maze (learning and memory)
  7. Y-maze (learning and memory)
  8. Radial Arm Maze (learning and memory)
  9. Novel object recognition (memory)

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