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Experimental models – In vitro models

  1. Perfusion system: Measurement of the release of sensory neuropeptides (substance P, CGRP and somatostatin and PACAP) from isolated organs, especially rat trachea with specific and sensitive radioimmunoassay techniques developed in our laboratory
  2. Murine and rat peritoneal macrophage cultures: Measurement of cytokine (especially IL-1-beta and TNF-alpha) production in response to LPS and PMA stimulation
  3. Primary culture of trigeminal neurones, astrocytes, peritoneal macrophages and receptor (TRPV1, TRPA1, sst1-5) expressing cell lines made in our laboratories: examination of ligand-receptor binding and receptor activation

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